Chromatrap provides a wide range of DNA purification methods and DNA purification kits. According to Chromatrap, “Depending on your tissue or cell samples, you can use a chemical treatment, enzymatic digestion or mechanical disruption to lysate the DNA. Removing contaminants from the nucleic acids and using a suitable buffer solution to precipitate the samples will aid the purification of the DNA. ”

Chromatrap recommend considering different criteria if you are trying to determine which purification method is the best for your DNA sample. The requirements could be anything from the source organism and starting material to the target nucleic acid, downstream application and, of course, the desired results.

There are also several genomic and plasmid DNA purification products designed to make the isolation process less time-consuming. These are suitable for a wide array of routine molecular biology applications, including high throughput screening.

Chromatrap state that ‘DNA purification kits currently on the market use much safer methods and can perform DNA isolation over a shorter period, about an hour or less.’ This is because cells are disrupted using detergents, chaotropic salts or alkaline denaturation, and the lysate which is created as a result is cleaned via filtration, centrifugation or magnetic clearing. The soluble portion of the lysate is then treated to obtain purified DNA.

Thus, the resulting high-quality DNA can be used for a range of downstream applications, including sequencing reactions and multiplex PCR.

So, how does DNA purification work?  Well, Chromatrap explains in detail the whole process. With your tissue or cell samples, you can use a chemical treatment, enzymatic digestion or mechanical disruption to lysate the DNA. Removing contaminants from the nucleic acids and using a suitable buffer solution to precipitate the samples will aid the purification of the DNA.

To determine which purification method is best for your DNA sample, you should consider different criteria, from the source organism and starting material to the target nucleic acid, downstream application and, of course, the desired results.

There are many genomic and plasmid DNA purification products designed to make the isolation process less time-consuming. These are suitable for a wide array of routine molecular biology applications, including high throughput screening.

Furthermore, Chromatrap explains what DNA extraction entails and what the DNA extraction kits are designed for. They are designed to help scientists isolate DNA from various types of samples and cells. While methods can somewhat vary between labs, extracting DNA from the cell nucleus commonly involves three key steps which are; cell breakdown, clearing out proteins, DNA precipitation and purification, and DNA quality check.

Chromatrap believes DNA purification to be essential for many reasons. First, ‘the fields of molecular biology, clinical research, genomics and biotechnology consider DNA purification an essential part of their scientific methods which helps them determine whether immediate and downstream applications have been successful.’

However, DNA purification also helps extract genomic and plasmid DNA in the sample quantities that your research requires. Therefore, this also extends their shelf-life and reduces the probability of error when it comes to research results, guaranteeing a high standard of from start to finish.

Get in touch with Chromatrap today if you would like to know more about the DNA purification methods or the DNA purification kits.  You can call Chromatrap today on +44 (0)1978 661144 and speak with one of the members of the team. Or you can go online to their website at https://www.chromatrap.com/dna-purification-products/ where there is plenty of information about the DNA purification kits and how to concentrate DNA.

Source: https://thenewsfront.com/chromatrap-provide-a-wide-range-of-dna-purification-methods-and-dna-purification-kits/